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fast gt sample loading reagent  (fluidigm)


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    Structured Review

    fluidigm fast gt sample loading reagent
    Fast Gt Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fast+gt+sample+loading+reagent/Fast+GT+Sample+Loading+Reagent/10__1200_slash_go___24___00512-31-29-34
    Average 93 stars, based on 14 article reviews
    fast gt sample loading reagent - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Purification:

    Article Title: Collagen Gene Polymorphisms Previously Associated with Resistance to Soft-Tissue Injury Are More Common in Competitive Runners Than Nonathletes
    Article Snippet: Fluidigm Protocol.Analysis was performed using a Fluidigm EP1 system and BioMark 192.24 GTDynamic Array Integrated Fluid Circuit (IFC) (Fluidigm, Cambridge, United Kingdom). .. Amixture of 2mL of GTXpressMasterMix (Applied Biosystems), 0.2mL of Fast GT Sample Loading Reagent (Fluidigm), 0.2 mL of nucleasefree H2O, and 1.6 mL of purified DNA (or H2O as control) was pipetted into each well of the IFC. .. In addition, 1.78 mL of TaqMan SNP Genotyping assay (Applied Biosystems), 1.78 mL of Assay Loading Reagent (Fluidigm), and 0.18 mL of ROX (Invitrogen, Paisley, United Kingdom) were combined per assay inlet.

    Article Title: Concussion-Associated Polygenic Profiles of Elite Male Rugby Athletes
    Article Snippet: Protocol one: Approximately 40% of samples were genotyped using a StepOnePlus (Applied Biosystems, Paisley, UK) as previously described [ ] with variations to thermocycling conditions depending on reagents used. .. Protocol two: Approximately 60% of samples were genotyped by combining 2 μL of GTXpress Master Mix (2×) (Applied Biosystems), 0.2 μL of 20× Fast GT Sample Loading Reagent (Fluidigm, Cambridge, UK), 0.2 μL of H 2 O and 1.6 μL of purified DNA. .. Furthermore, 1.78 μL of assay (20×) (Applied Biosystems), 1.78 μL of 2× Assay Loading Reagent (Fluidigm) and 0.18 μL of ROX reference dye (Invitrogen, Paisley, UK) were combined.

    Article Title: Variants within the MMP3 and COL5A1 genes associate with soft tissue injury history in elite male rugby athletes.
    Article Snippet: Protocol one: Approximately 20 % of the DNA samples were genotyped via real-time PCR using a StepOnePlus (Applied Biosystems, Paisley, UK) as previously described,31 with adjustment of thermocycling conditions depending on reagents utilised. .. Protocol two: Approximately 80 % of the DNA samples were genotyped by combining 2 μL GTXpress Master Mix (Applied Biosystems), 0.2 μL Fast GT Sample Loading Reagent (Fluidigm, Cambridge, UK), 0.2 μL H2O and 1.6 μL of purified DNA, for samples derived from blood and saliva. .. Furthermore, 1.78 μL assay (Applied Biosystems), 1.78 μL Assay Loading Reagent (Fluidigm, Cambridge, UK) and 0.18 μL ROX reference dye (Invitrogen, Paisley, UK) were combined per assay inlet.

    Article Title: Gene variants previously associated with reduced soft tissue injury risk: Part 1 - independent associations with elite status in rugby.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 2 μL GTXpress Master Mix (Applied Biosystems), 0.2 μL Fast GT Sample Loading Reagent (Fluidigm, Cambridge, UK), 0.2 μL H2O and 1.6 μL of purified DNA, for samples derived from blood and saliva. .. Furthermore, 1.78 μL assay (Applied Biosystems), 1.78 μL Assay Loading Reagent (Fluidigm) and 0.18 μL ROX reference dye (Invitrogen, Paisley, UK) were combined per assay inlet.

    Article Title: Gene variants previously associated with reduced soft-tissue injury risk: Part 2 - Polygenic associations with elite status in Rugby.
    Article Snippet: Part 1 of this genetic association series highlighted several genetic variants independently associated with elite status in rugby.. However, it is highly likely that the genetic influence on elite status is polygenic due to the interaction of multiple genes.. Therefore, the aim of the present study was to investigate whether polygenic profiles of elite rugby athletes differed from non-athletes utilising 13 genetic polymorphisms previously associated with tendon/ligament injury.

    Control:

    Article Title: Collagen Gene Polymorphisms Previously Associated with Resistance to Soft-Tissue Injury Are More Common in Competitive Runners Than Nonathletes
    Article Snippet: Fluidigm Protocol.Analysis was performed using a Fluidigm EP1 system and BioMark 192.24 GTDynamic Array Integrated Fluid Circuit (IFC) (Fluidigm, Cambridge, United Kingdom). .. Amixture of 2mL of GTXpressMasterMix (Applied Biosystems), 0.2mL of Fast GT Sample Loading Reagent (Fluidigm), 0.2 mL of nucleasefree H2O, and 1.6 mL of purified DNA (or H2O as control) was pipetted into each well of the IFC. .. In addition, 1.78 mL of TaqMan SNP Genotyping assay (Applied Biosystems), 1.78 mL of Assay Loading Reagent (Fluidigm), and 0.18 mL of ROX (Invitrogen, Paisley, United Kingdom) were combined per assay inlet.

    Derivative Assay:

    Article Title: Variants within the MMP3 and COL5A1 genes associate with soft tissue injury history in elite male rugby athletes.
    Article Snippet: Protocol one: Approximately 20 % of the DNA samples were genotyped via real-time PCR using a StepOnePlus (Applied Biosystems, Paisley, UK) as previously described,31 with adjustment of thermocycling conditions depending on reagents utilised. .. Protocol two: Approximately 80 % of the DNA samples were genotyped by combining 2 μL GTXpress Master Mix (Applied Biosystems), 0.2 μL Fast GT Sample Loading Reagent (Fluidigm, Cambridge, UK), 0.2 μL H2O and 1.6 μL of purified DNA, for samples derived from blood and saliva. .. Furthermore, 1.78 μL assay (Applied Biosystems), 1.78 μL Assay Loading Reagent (Fluidigm, Cambridge, UK) and 0.18 μL ROX reference dye (Invitrogen, Paisley, UK) were combined per assay inlet.

    Article Title: Gene variants previously associated with reduced soft tissue injury risk: Part 1 - independent associations with elite status in rugby.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 2 μL GTXpress Master Mix (Applied Biosystems), 0.2 μL Fast GT Sample Loading Reagent (Fluidigm, Cambridge, UK), 0.2 μL H2O and 1.6 μL of purified DNA, for samples derived from blood and saliva. .. Furthermore, 1.78 μL assay (Applied Biosystems), 1.78 μL Assay Loading Reagent (Fluidigm) and 0.18 μL ROX reference dye (Invitrogen, Paisley, UK) were combined per assay inlet.

    Article Title: Gene variants previously associated with reduced soft-tissue injury risk: Part 2 - Polygenic associations with elite status in Rugby.
    Article Snippet: Part 1 of this genetic association series highlighted several genetic variants independently associated with elite status in rugby.. However, it is highly likely that the genetic influence on elite status is polygenic due to the interaction of multiple genes.. Therefore, the aim of the present study was to investigate whether polygenic profiles of elite rugby athletes differed from non-athletes utilising 13 genetic polymorphisms previously associated with tendon/ligament injury.



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